Sialic Acids on the Plasma Membrane of Cultured Human Lymphoid Cells
نویسندگان
چکیده
From 61 to 92% of the total sialic acid of a variety of human lymphoid cell lines maintained in tissue culture is present on the cell surface as measured by its susceptibility to cleavage by Clostridium perfringens neuraminidase. These cells contain from 1.22 x 10(8) to 6.99 x 10(8) molecules of surface sialic acid per cell. In synchronized cultures synthesis of surface sialic acid occurs only during a limited time in the late G(2) phase of the cell cycle. The amount and density of surface sialic acid vary considerably throughout the cell cycle.
منابع مشابه
The Effect of Sialic Acid on miR-320a and let-7e Expression in Human Glial Cell Line
Introduction: Sialic acid plays pivotal roles in various critical physiological events at molecular and cellular levels and also pathological processes. Changes in sialic acid concentration are observed in many pathological processes; for example, there are some available data on the evaluated level of sialic acid and neurodegenerative prevalence. It can be proposed that sialic acid can play a ...
متن کاملSelective radioactive labeling of cell surface sialoglycoproteins by periodate-tritiated borohydride.
Low concentrations of sodium metaperiodate induce specific oxidative cleavage of sialic acids between carbon 7 and carbon 8 or carbon 8 and carbon 9. The aldehydes formed can easily be reduced with NaB3H4 to tritiated 5-acetamido-3,5-dideoxy-L-arabino-2-heptulosonic acid or 5-acetamido-3,5-dideoxy-L-arabino-2-octulosonic acid. At 0 degrees, the periodate anion penetrates the cell plasma membran...
متن کاملThe effect of virulence on cultivation of Theileria annulata strains in lymphoid cells which have been cultured in suspension
متن کامل
An alarming biochemical side effect of Teucrium polium on the plasma membrane alkaline phosphatase of k562 cell line
The unexpected enhancement of alkalinee phosphatase (ALP) activity in the serum of a group of STZ-diabetic rats recieving the Teucrium polium extract (with hypoglycaemic property) may be attributed to its probable side effects. In order to get a more precise knowledge about this subject, we evaluated the effect of T. polium extract on the plasma membrane ALP activity of K562 cell line. Our data...
متن کاملAn alarming biochemical side effect of Teucrium polium on the plasma membrane alkaline phosphatase of k562 cell line
The unexpected enhancement of alkalinee phosphatase (ALP) activity in the serum of a group of STZ-diabetic rats recieving the Teucrium polium extract (with hypoglycaemic property) may be attributed to its probable side effects. In order to get a more precise knowledge about this subject, we evaluated the effect of T. polium extract on the plasma membrane ALP activity of K562 cell line. Our data...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 53 شماره
صفحات -
تاریخ انتشار 1972